RNA

Part:BBa_K3752000:Design

Designed by: Tudor Onose   Group: iGEM21_Warwick   (2021-10-20)


OXA-48 Parent cgRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RNA had to be engineered to have a series of domains which would cause the dCas9 binding handle to be misfolded in its native state. When the OXA-48 is present however, the two mRNAs anneal in such a way that the dCas9 binding handle is folded correctly.


Source

Designed

References